溴丙酰螺的致突变性研究

STUDIES ON THE MUTAGENICITY OF SPIROBROMIN

溴丙酰螺为河南焦作制药厂研制的抗肿瘤药物，本文报道它在３个短期试验中的研究结果。Ａｍｅｓ试验的浓度为１，１０，１００，１０００和５０００ｕｇ／ｋｇ，结果为阴性，小鼠骨髓微核试验的剂量为１１６８．０，５８４．０，２９２．０ｍｇ／ｋｇ，１次腹腔注射，试验结果为阳性。中国仓鼠肺细胞染色体畸变试验的浓度为７６０，３８０，１９０ｕｇ／ｍｌ。溴丙酰螺在有活化条件的情况下对中国仓鼠肺细胞染色体有明显的损伤作用，并有剂量反应关系，故可判断为阳性。

Mutagenicity of spirobromin(N,N3 ─di─(β─Bromopro─pionyl)─N,N─Dispirotriperazinium dichloride),an antitumor chemical was tested for the accordance tothe requirement of Chinese regulation of new Drug Approval. Ames test with TA97,TA98, TA100 and TA102,no increase of revertant colonies in all test strains over back-ground level was observed. The micronucleus rates of polychromatocyte in bone marrow ofmice received (ip)dosages of 1168, 584 and 292 mg/kg were 29.5,28.0 and 22.5% re-spectively. That is weak positive. Rates of chromosome aberration in CHL were 90,49and 24%(24h with S9) with the dosages of 760, 380 and 190 ug/ml in culture respective-ly, Result indicated that Spirobromin significance damage chromosome.It is concludedthat Spirobromin is mutagenic.