三种毒物对小鼠受精卵染色体畸变的影响

THE EFFECTS OF THREE GENOTOXIC CHEMICALS ONTHE CHROMOSOMES IN MOUSE ZYGOTES

本文采用小鼠受精卵染色体分析方法，对敌枯双、重铬酸钾、二硫化碳三种毒物的生殖细胞遗传物质诱变作用进行了评价，结果证明阴性对照组染色体数目畸变细胞率为９．Ｏ％，与各染毒组比较均有显著差异，敌枯双组为５１．８％，（Ｐ＜０．０１）；重铬酸钾组为３３．８％，（Ｐ＜０．０１）；二硫化碳（１０ｍｇ／ｍ３）组为２２．８％，（Ｐ＜０．０１），（１００ｍｇ／ｍ３）组为２｝８％，（Ｐ＜０．０１）。

In this paper,a new method for chromosome preparetion of zygotes from mouse was described.Mutagenesis of N, N-methyoer -bis (2-amiog-1,3,4,-thiadiazole)(MATDA),Potassium dichromate(PD),Carbon disulflde(CD )damaging tochromosomes in the zygotes was eval uated hy the method.The result shown that theincidence of zyilotes with abnormalities of chromosomal numbers of control group was 9.0%,The incitience of four groups exposed to MATDA( 2mg/kg,wt),PD(4. 8mg/kg.wt), CD(10mg/m3) CD(100mg/m3)were 5l.8%(P<0.01),33.8%(P<0.01),22.8%(P<0.01),29.8%(P<0. 01)respectively,suggested that the genotoxic cliemicals aremutagenic effects on chromosomes of mouse zygotes.