DDNAQ致突变作用的研究

STUDY ON MUTAGENICITY OF 1 2,4 DINITRO PHENOXY 5 3,4 DINITRO PHENOXY 4,8 8 DINITROANTHRAQUINONE(DDNAQ)

本研究对某厂１２２名接触１─〔２．４─二硝基〕苯氧基—５—〔３．４—二硝基〕苯氧基—４．８—二硝基蒽醌（ＤＤＮＡＱ）（车间空气粉尘浓度为０．１６ｍｇ／ｍ３）的工人和某厂１００名非接触工人进行了口腔粘膜上皮细胞微核检查，发现接触组微核人员出现率和平均微核率分别为７６．２２％和２％，显著高于非接触组相应的３０．００％（Ｐ＜０．０１）和０．４％（Ｐ＜０．０１）。是时，上述接触组和非接触组各１２名工人进行了中性白细胞ＤＮＡ含量测定，接触组（６００个细胞）ＤＮＡ含量为３．６７±０．３０，与非接触组（６００个细胞）的３．０２±０．１８相比较，差别显著（Ｐ＜０．０１）。此外，动物实验结果表明，小鼠受精卵染色体数目畸变细胞率，染毒组（０．８ｍｇ／ｋｇ·ｗｔ）为１８．７５％，显著高于对照组的８．７４％（Ｐ＜０．０５）；金黄地鼠胚胎早期培养细胞转化试验，染毒组（０．１ｕｇ／ｍｌ，１．０ｕｇ／ｍｌ转化克隆／总克隆为４／４６７、３／４４２（空白对照组为０／５０８），呈阳性结果。上述结果说明ＤＤＮＡＱ对遗传物质存在作用，应加强职业性接触工人卫生防护工作，保护工人及其后代的健康。

The micronuclei of exfoliated buccal mucosa cells in 122 workers exposed toDDNAQ(dust concentration in workshop was 0.16mg/m3)and l00 non exposed wereexamined. Positive rate of mucronuclei and average rage of micronuclei in exposed groupwere 76.22%,0.2%respectively,in non exposed were 30.00%,0.4%respectively. Thetwo rates in group exposed to DDNAQ were very higher than those in non exposed(P<0.01,P <0.01). At the sarne time,DNA content in 600 neutrocytes of 12 workers eitherof group exposed to DDNAQ and control were quantitated.DNA content in group exposedto DDNAQ was 3.67±0. 30, in control was 3.02±0.18.the results showed statistical difference(P<0.01).In addition,chromosomal abberational cell rate in mouse zygotesfor group exposed to DDNAQ(0.8mg/kg·wt)was 18.75%,it was higher than 8. 74% incontrol( P<0.05).In cell transformation test of golden hamster embryo cells cultured atearly Stage,transformed clones and general clones in groups exposed to DDNAQ(0.1g/ml,1.0g/ml)were 4/467,3/442 respectively,in control was 0/508),the results diagnosedin groups exposed to DDNAQ were positive.The results demonstrated that DDNAQeffected on genetic material.