摘要
我们以非组生白质(NHP)抗体作为抗癌药物平阳霉素(A5)的载体,制备NHPIgGA5交联物。应用(1)细胞培养隔日计数示生长曲线;(2)以Feulgen法示细胞DNA相对含量;(3)3H-TdR掺入实验、比较了NHPIG-A5、NHPIgG及A5对人食管癌细胞株ECa109的体外抑制效应,四者比较,NHPIgG-A5人食管癌Eca-l09细胞株有较强的抑制效应。
Bleomycin A5 was conjugated to anti-NHPlgG by sodium periodateoxidation method.The cytostatic effect of NHP、 NHPIgG-A5 conjugate. free A5 andCEAIgG-A5 conjugate on the human esophageal cancer cell line: ECAa-109 cell, wasstueied : ① the growth curve of cells, ② the relative content of DNA of cells; ③ inc-orporation of 8H-TDR into cells. The results Showed that the four agents expressedcytostatic activity against the Eca-109 cell line. The most effective agent was NHP-IgG-A5. It is concluded that the cytostatic effect of NHPIgG-A5 conjugate was super-ior to that of CEAIgG-A5 conjugate, free A5 and NHPIgG alone.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
1994年第3期217-220,共4页
Chinese Journal of Cancer
关键词
ECA-109
细胞株
平阳霉素
食管肿瘤
Nonhistone protein(NHP) conjugate Growth curve of cell DNA relative content 3H-TDR incorporation (Department of Histology and Embryology, Shanghai University, shanghai 200032 )