氮芥抗药性人早幼粒白血病HL_60细胞亚系的建立及其生物特性

ESTABLISHMENT OF NITROGEN MASTARD-RESISTANT HUMAN LEUKEMIA HL60 SUBLINE AND ITS CHARACTERIZAIT0N

用逐步递增药物浓度和软琼脂细胞克隆技术，药物连续作用１４个月后获得氮芥（ＨＮ２）抗药性人早幼粒白血病ＨＬ６０细胞亚系，抗件达５～７倍（ＨＬ６０／ＮＨ２６），在无ＨＮ２培养液中５倍以上抗性维持１０个月以上，抗性细胞亚系的生物学特性除倍增时间稍长和克隆形成率略低外其生长曲线，细胞周期细胞ＤＮＡ指数，形态学，组织化学和染色体分析均与亲代细胞相似。

BY continuously exposing cells to gradually increased concentration ofnitrogen mustard(HN2 ) and cell colonal technique,a resistant to HN2 HL60 human leu-kemia cell subline was established over a period l4 months. This subline has 5～7 foldsresistance to HN, and was stable when maintaining in l2μmol/ ml HN2 in cultuire,and the 5 folds resistance was maintained to a period Of l0 months without HN2. Thebiological characterization of this subline,including growth curve,plate efficiency,dou-bling time,istological morphology,cell cycle(FCM) , histological chemistry and chrom-some analysis was examined.The results showed that most of the above parameters ofHN2 resistant HL60 subline were as the same as those of its parent cell line,except that the plate efficiency was lower and doubling time was relatively longer than thatat of its parent cell line.