NSE—McAb双位点夹心ELISA的建立及在小细胞肺癌中的应用研究

ESTABLISHMENT OF TWO-SITE SANDWICH ENZYME LINKED IMMUNOSORBENT ASSAY OF NSE-MCAB AND ITS APPLICATION IN THE SMBALL CELL LUNG CANCER

本文在自制８株神经原特性烯醇化酶单抗（ＮＳＥ—ＭｃＡｂ）的基础上，对４株ＭｃＡｂ分别标记辣根过氧化物酶（ＨＲＰ），经多次方阵排列滴定，建立了ＮＳＥ—ＭｃＡｂ双位点夹心ＥＬＩＳＡ。通过对２３例小细胞肺癌（ＳＣＬＣ），３２例非小细胞肺癌（ＮＳＣＬＣ），２２例肺良性疾病（ＢＰＤ）和正常对照血清中ＮＳＥ检测表明，对ＳＣＬＣ诊断的阳性率为８２．６％，特异性为９７．８％。对ＳＣＬＣ的临床分期和判断预后也有一，定的帮助。

Two-site sandwich enzyme liked immunosorbent assay(ELISA)was established and multiple check- board titration was performed,using H R P separ-tely labelled 4 McAbs among the 8 McAbs purified by our ladoratory.We measuredthe serum Neuro-specific enolase(NSE),levels in 23 patients with SCLC, 32 patientswith non-small cell lung cancer(SCLC),22 patients with benign puinlonary disease(BPD) and normal controls(NC).The positive rate of detection was 82.6 % and speci- ficity was 97.8%for SCLC.Two-siste sandwich ELIISA may be valuabe for clinicalstaging and evaluating prognosis in patients with SCLC.