生物素标记探针原位DNA分子杂交中的假阳性

THE FALSE-SIGNALS IN DNA-DNA IN SITU HYBRIDIZATION WITH BIOTINYLATED PROBE

以生物素标记的ＥＢ病毒ＢａｍＨＩ酶切ｗ片段为探针，原位检测人鼻咽癌等石蜡切片中的ＥＢ病毒ＤＮＡ，结果发现：在部分标本的癌细胞胞浆中和非癌的鼻咽增生上皮细胞中，鼻咽粘膜下小腺体及唾液腺细胞浆中，存在内源性的亲和素结合活性，因此产生假阳性信号。文章介绍了设立相邻切片的无探针对照来区分真伪的方法，并对生物素标记探针原位杂交技术的适用范围进行了讨论。

In the experiment of in situ detection of EBV-DNA on routine paraffin sections of nasopharyngeal carcinoma biopsies’it was discovered that some cells in some biopsies showed endogenous avidin binding activity which resulted in pseudo-signals.The method used to distinguish the false and true positive signals was introduced and the applicable scope of biotinylated probe on in situ hybridization was discussed.