大肠癌细胞转铁蛋白受体表达的临床意义

CLINICAL SIGNIFICIANCE OF TRANSFERRIN IN RECEPTOR EXPRESSION ON CARCINOMA CELL OF THE COLON

应用抗人类细胞转铁蛋白受体（ＴＦＲ）的单克隆抗体（ＯＫＴ９），以冰冻切片ＡＢＣ法研究了４１例大肠癌、１９例癌旁组织、２５例正常人大肠粘膜组织ＴＦＲ的表达。结果表明：癌、癌旁及正常人大肠粘膜组织ＴＦＲ染色阳性率依次为８２．９％、８４．２％和５２％，癌及癌旁组织ＴＦＲ染色阳性率及染色强度均显著高于对照组（Ｐ＜０．０５，Ｐ＜０．００５）。正常人大肠粘膜腺体及形态学正常的癌旁腺体ＴＦＲ染色以细胞基底部及侧部为主，呈线形或弧形；不典型增生及癌细胞ＴＦＲ染色以细胞膜为主，大多数呈棕色环形。本研究结果从对照组→癌旁正常粘膜→不典型增生→癌，其ＴＦＲ表达依次增加，支持大肠癌发生和演变规律，即正常粘膜→移行粘膜→癌。动态检测大肠粘膜活检标本ＴＦＲ表达有可能用于监测细胞癌变，ＴＦＲ染色分布的特点，为病理工作者应用光镜鉴别形态正常腺体细胞与不典型增生和癌细胞提供新的指标，可望对大肠癌早期诊断具有意义。

The authores studied the expression of transferrin receptors(TFRs)on frozen sections of normal human coion mucosa(n=25),tissues adjacent to colon ca-ncer(n=19)and colon cancer(n=41)by the monocolonal antoibody OKT9 and ABCimmunohistochemical staining. The positive rate of TFR staining was 52%,84.2%and 82.9%in the abovementioned three kinds of tissues respectively,The positive rateof TFR staining and the intensity in tissues adjacent to cancer and in the cancer weresignificantly higher than those in mormal human colon mucosa(p<0.05,P<0.00 5)TFRs were demonstrated on some normomorph mucosa glands(from normal persons andpatients)predominantly in the basal-lateral area,and on the whole cell membrane ofdysplastic and carcinoma cells. The results support the hypothesis that normal colonmucosa evolve into carcinoma through transitional mucosa. Periodic biopsy tests forTFR of the colon epithelium may be useful for monitoring the carcinogenesis of theepithelium. The TFR staining features of different types of tissues may help patholog-ists to distinguish normorph cells from dysplastic and carcinoma cells and to diag-nose the colon tumors earlily.