视黄酸对斑马鱼胚胎发育的影响

The Effects of Retinoic Acid on Zebrafish Embryo

作者首次通过Ｂｒｄｕ－ａｎｔｉ－Ｂｒｄｕ法标记处于Ｓ期细胞核等系列实验表明，视黄酸对斑马鱼早期胚胎的中枢神经系统影响较大，主要表现为由前胸缺损而引起的小头畸形、无眼或无心脏，但后脑及脊髓部分的原始反射弧仍存在，而对尾芽只作用于顶端生长区．另外，利用低浓度的视黄酸还诱导出了过早出现的爪蟾前肢及火鲑鱼晚期尾芽胚的腹鳍，说明视黄酸对软骨的发生依浓度不同而有促进与抑制两方面的作用。

Retinoic acid (RA) is well known as a biologically active form of vitamin A and a teratogen. It affects embryonic development, differentiation and tumour cell growth. The purpose here was to begin analysing the effects of RA on CNS and tailbud development in zebrafish (Brachydanio revio ). The effect of RA on zebrafish embryo is dose-dependent at wall as stage dependent. The affected embryos show microcephaly (no anteriorbrain), no eye or no heart formation and the tailbud formatiod is suppressed. but the primary reflex pathway of posterior brain and spinal cord survive. The identification of Brdu-labelled nuclei in S-phase by Brdu-anti-Brdu method suggest that RA inhibit the formation of tailbud growth zone and the embryobrain after 16 hour is unaffect by RA treatment, othersides, RA is both promote and inhibit cartilage formation depend on the conditions.