喉癌与人类乳头状瘤病毒相关性的研究

Study on relationship between laryngeal carcinoma and human papillloma virus

本研究采用分子生物学核酸斑点杂交技术，以［α－３２Ｐ］一ｄＣＴＰ标记的人类乳头状瘤病毒（ＨＰＶ）１６型ＤＮＡ为探针，分别对３９例喉癌组织、１０例正常喉粘膜及１例喉脂肪肉瘤进行了检测，并对ＨＰＶＩ６的感染率与喉癌的临床分型、临床分期、病理分化程度及性别的相关性进行研究。结果表明，ＨＰＶ１６的感染程度与喉癌的临床分型及分期有关（Ｐ＜０．０５），而与病理分化程度及性别无关（Ｐ＞０．０５）。故我们认为ＨＰＶ与喉癌的发生有一定的关系，ＨＰＶｌ６在喉组织中的存在属于病理性侵袭，致喉癌的危险度较高。

By using a一32 P一labelled DNA of human papilloma virus(HPV)type l6 as theprobe,we examined 39 specimens of laryngeal carcinoma(the cancer group)’10 of normal la-ryngeal epithelium(the con trol grou p) and l of laryngeal fat sarcoma with dot blot hybrldiza -tion.The results showed that HPV 1 6 DNA sequ ences were detcctedin 23 out of 39 laryng ealcarcinoma (59.0%)and l ou t of l0 normal laryngeal epitheliu m(10.O%), with the differenceof significance statistically(P<0. 0l).We also detected HPV I6 DNA sequen ce in the laryngealfa t sarcoma.It was revea led that the severity of HPV 16 infection might be related to the clin icaltypes and stages of laryngeal carcinoma,but not to the sex and the histological differentiation oftumor. These re ults suggest that HPV infection is certainly related with the developm ent of laryngeal carcinoma; HPV 16 in fectioin is a pathogenic invasion an d may be a high risk for the developm en t of laryn geal can cer.We also find that HPV 1 6 i11fection is closely associated withsmoking, so that a synergistic in teraction between HPV infectioi1 and smoking may be of someimportance in the development of laryngeal cancer.