人和大鼠输精管平滑肌和神经的形态学研究

A MORPHOLOGICAL STUDY ON THE MUSCULATURE AND INNERVATION OF THE VAS DEFERENS IN RAT AND MAN

本实验用组织化学、电镜和生化测定方法研究了人和大鼠输精管的平滑肌和神经分布。结果表明;大鼠输精管的神经密度明显高于人,轴索数/100个肌细胞断面,大鼠是118,人是315肌-神经间隔,大鼠是25nm左右,人是100nm左右;肌细胞外间隙(％),大鼠是12.9,人是35.8;肾上腺素能神经支配占优势,乙醛酸诱发荧光法显示大鼠输精管肌层内有致密的肾上腺素能神经纤维,ACh阳性反应神经较少,主要分布于固有层,人输精管中上述两种神经均较少;每克湿组织中NE含量(ng)大鼠约为人的10倍左右(大鼠:2369.35±820.27,人:294.66±83.90);电镜观察,SGV型神经终末支多见。

By using histochemical technique, electron microscopic observation and biochemical measurement, a morphological study of musculature and innervation of the vas edeferens of rat and man was carried out. The results revealed that the nervedensity in rat was higher than that in man; number of axons/per 100 cross sections of smooth muscle cell: rat, 118, man, 31; neuromuscular contacts. rat, about 25 nm, man about 100 nm; extracellular space(%): rat, 12.9, man, 35.8. Besides, the adrenergic innervation played a dominant role in rat vas deferens. Glyoxylic acid induced fluorescent method showed that numerous adrenergic nerves existed in the muscle layer of rat vas defferens while a smaller number of AChE positive nerves was observed in the lamina propria. The content of NE(ng)/ gram wet weight of rat vas deferens was about 10 times as that of man; rat, 2389.35±820.27, man, 294.66±83.90. Under elcctron microscopy, the varicosities containing small granular synaptic vesicles were often seen. The physiological significance of the differences in musculature and innervation between rat and man is discussed.