摘要
ＳＰＦ鸡动物实验室的建立及其实验应用范书曦，周蛟，郭志弘，姚炜光，闫乃颐，苏凤林，刘有昌北京市农林科学院畜牧兽医研究所前言随着科研水平的不断提高，对实验动物以及饲养实验动物的环境、设施的要求也有了全新的内容和根本的差别。我所根据“北京市实验动物饲养与...
The key concept involved in the basic design is the
isolation against contaminants while assuring necessary opera- tions
of researchers and transport of materials required by the
experimentation.On the one hand,most parts of the building are well
sealed and air filters and various disinfectant measures are
interposed at various points to cut off any contamitant that might
get in.On the other hand,several channels are provided to insure the
smooth but supervised flow of workers and materials in- volved.Most
notably,filtered clean air forms nearly closed circulation in
isolation room,with turnover amount lost via isolators and incubation
room which are replenished from outside through entrance.This means
both insurance of clean air and saving of en- ergy.Besides,a
relatively higher or lower air pressure is maintained in a number of
special isolators to prevent,respectively,the clean object(eg.healthy
control)from being contaminated or the unclean object(eg.infected
chicken) from making contamination of outside objects And our
experience has proved the efficacy of effectively filter out most
particles arising from within such as downs and feed debris, All the
experimental materials are forced to make a one-way flow.With the
wastes disposed at the end. Power supply is guaranteed by double
lines and a selfdesigned DC plonum fan will be set in motion
automatically in case of power outage.In addition,inner telephones
are installed to insure timely communication which spares the need of
personal movement the might cause contamination.Technical assessment
has found that dust content.the noise level and indoor air pressures
all reach 10 the standard.But more significantly,actual experiments
using this superclean laboratory hasproved its real value.For
example.sharp contrast was demonstrated between 100%protection in
immunized chicken challenged with100 BID 50 of IBDV variants and
100%infection in unimmunized ones which were reared in different
isolators in the same room under the same conditions,a fact that
demonstrated perfect isolation among the isolators.
