摘要
用胃转移性腺癌细胞系ＳＧＣ７９０１免疫ＢＡＬＢ／Ｃ小鼠，取脾与ＳＰ２／０骨髓瘤细胞融合，筛选出４株抗胃癌单克隆抗体，分别命名为ＢＭＧ１，ＢＭＧ２、ＢＭＧ３和ＢＭＧ４。经间接细胞免疫荧光和ＡＢＣ免疫组化染色鉴定，这组单抗与胃癌细胞系和胃癌组织有高的反应性，其中ＢＭＧ１、ＢＭＧ３与中分化腺癌及低分比腺癌的反应性分别为８４．６％和８３．３％；与正常胃粘膜腺体不反应；与部分异型增生腺体呈阳性反应。初步认为，此组单抗在胃癌的组织学和体液诊断方面有一定应用价值。
our monoclonal
antibodies disignated BMGs were generated by immunizing BALB/c mice
with the SGC7901,A gastric adenocarcinoma cell line. 123 cases of
gastric specimen were studied by immu- nohistochemistry. BMG1 and
BMG4 reacted with 77.8%and 81.3%of the well differentiated adenoca-
cinoma,moderately differentiated adenocarcinoma and the reactivity of
84.6%and 66.7%,respecti- vely;83.3%of poorly differentiated
adenocarcinoma and 71.4%of mucinous adenocarcinoma were stained by
BMG3.The MAbs did not react with normal gastric tissues.The
corresponding antigens were consi- dered as glycoproteins expressed
on the cell membrane.
出处
《北京医科大学学报》
CSCD
1994年第6期447-449,共3页
Journal of Peking University(Health Sciences)
