摘要
以Ｒｂ基因ｃＤＮＡ３．８Ｋｂ片段做探针，经非放射性地高辛—ｄＵＴＰ及放射性同位素α－３２Ｐ－ｄｃＴＰ两种标记方法标记，对６株人胃癌细胞株的ＤＮＡ进行点杂交和Ｓｏｕｔｈｅｒｎ印迹杂交，显示５株细胞Ｒｂ基因完全缺失，１株不全缺失伴突变。
We
examined the disorders of RB gene in six human gastric cancer cell
lines with Dot and Southern blot hvbridization. The probe,Rb cDNA 3.8
kb,was labelled with nonradioactive digoxin-dUTP and radioactive
isotope α-32P-dCTP respectively. With two kinds of hybridization
methods,complete dele- tion of Rb gene was detected in five cell
lines,and partial loss and mutation of this gene was found only in
MGC-803 cell line
出处
《北京医科大学学报》
CSCD
1994年第2期125-127,共3页
Journal of Peking University(Health Sciences)
基金
八五攻关基金
关键词
胃癌细胞株
RB
抗癌基因
点杂交
Human gastric cancer cell lines
Rb
antioncogene
Dot blot hybridization
Southern blot hybridization
