人白细胞介素－9cDNA的PCR扩增及其在大肠杆菌的表达

PCR AMPLIFICATION OF HUMAN INTERLEUKIN-9cDNA AND ITS EXPRESSION IN E COLI

利用ＰＣＲ技术从人外周血Ｔ细胞ｃＤＮＡ文库中扩增出人ＩＬ－９的ｃＤＮＡ，将其连入大肠杆菌表达载体后，可在大肠杆菌高效表达出人重组ＩＬ－９融合蛋白，表达量占菌体蛋白的２５％左右，有明显的刺激正常人骨髓细胞集落形成的活性。

The cDNA coding region for human interleukin-9(IL-9)Was amplified from a human T cell cDNAlibrary with PCR technique. The PCR products were subcloned into a E. coli expression、vecor and human recombinant IL-9 fusion protein was expressed with high ejjiciency in E. coli .Up to 25%of the total bacterial proteins was the recombinant IL-9. Biological activity analysis showed that the exp- ressed IL-9stimulated increased colony-formation of normal human bone marrow cell.