中国人群HLA－B＿（27）等位基因亚型与强直性脊柱炎的相关性研究

ANKYLOSING SPONDYLITIS AND HLA-B27 SUBTYPES: ADISEASE-RELATED RESEARCH INCHINESEHAN NATIONALITY

利用聚合酶链式反应和顺序特异性寡核苷酸探针斑点杂交方法，对７８例中国汉族强直性脊柱炎（ＡＳ）病人ＨＬＡ－Ｂ２７，基因分析结果显示，该基因与ＡＳ易感性显著相关，在中国汉族ＡＳ病人中至少存在两种亚型，其中Ｂ２７４０亚型频率最高（占５４．８％），其次Ｂ２７０５（占４１．１％）。实验结果表明，不同人种ＡＳ患者的Ｂ２７亚型分布频率不同；与强直性脊柱炎相关的可能是各亚型共有的序列特征，即Ｂ２７特异性抗原决定基而与各亚型特异性序列特征无关。

LA- B27 represents a family of closely related antigens. Six alleles differing in a limited numberof nucleotide substitutions have been described (B2701- B2706). Different specific residues are clusteredin a l and a 2 domains. So far only Caucasian and Gambians( Africa) had been investlgated. Wesubtyped HLA-B27 in HLA-B27 positive ankyiosing spondylitis patients of Chinese Han nationality forthe first time. Polymerase chain reaction was designed to amplify regions of class l(exons 2 and 3).Amplified sequences were tested with eight sequence- specific oligonucleotides to distinguish all HLA- B27 subtypes.lt was found B2704 was the most common subtype existing in 54.8%,and B2705 inapproximatcly 41.1%. We believe the above results imply that the prevalences of the various subtvpesof HLA- B27 vary in different AS populations; the HLA- B27s pecific resldues that are shared by thesubtypes(B2701-B2706)may be involved in the pathogenesisof AS