用电镜原位杂交法定位急性白血病免疫球蛋白重链基因重排

LOCALIZTION OF IMMUNOG LOBUL IN HEAVYCHAINGENE REARRANG EMENTIN CASES WITHACUTE LEUKEMIAU SING ELECTRON MICROS COPIC IN SITUHY BRIDIZATION

采用电镜原位杂交法，观察６例急性白血病免疫球蛋白重链（ＩｇＨ）基因重排，结果发现，５例急性淋巴细胞白血病（ＡＬＬ）阳性，表明均为Ｂ－ＡＬＬ；１例急性粒单细胞白血病（ＡＭＬ－Ｍ４）阴性。在阳性细胞核内，代表ＩｇＨ基因重排的胶体金颗粒成群分布，该技术把核酸探针杂交与超微形态学相结合，在基因水平确定白血病细胞类型，尤其对缺乏特异免疫表型的白血病更有应用价值。

Immunoglobul n heavy chain(IgH)gene rearrangements were observed in six patients with acute leukemia using electron microscopic in situ hybridization,The results indicated that five cases with acute lymphocytic leukemia (ALL) were positive,and one case with AML- N4 was negative. Five caseswith ALL were B- ALL. In positive nuclei,colloid gold particles which represented IgH gene rearran- gem ent distrlbuted in clusters. This technique combined nucleic acid probe hybridization with ultrastru-ctural morphology,and it was possible to identify leukemic cells at the gene level,probably more imp-ortant in acute leukemia cases that lack special immunophenotype.