过氧化苯甲酰对小鼠皮肤表皮鸟氨酸脱羧酶mRNA表达的影响

EFFECT OF BENZOYL PEROZIDE ON THE EXPRESSION OF ORNITHINE DECARBOXYLASE mRNA IN MOUSE EPIDERMIS

过氧化苯甲酰是一种可产生自由基、具有促癌活性的化合物。Ｎｏｒｔｈｅｒｎ印迹分析研究表明，ＢＰＯ在４０ｍｇ一次局部涂用于小鼠皮肤后，皮肤表皮鸟氨酸脱羧酶（ＯＤＣ）ｍＲＮＡ水平在涂用ＢＰＯ后６小时左右升至最高，而ＢＰＯ多次涂用，也可增加ｍＲＮＡ水平，并以第６小时最明显。提示，ＢＰＯ可能通过促进ＯＤＣｍＲＮＡ表达、增加ＯＤＣ酶量以诱导ＯＤＣ活性。

t has been proved that benzoyl peroxide (BPO),a free radical- generatingxompound,hastumor promoting activity BPO topically applied on mouse skinsignificantlyinducedepidermalornithinedecar-boxylase(ODC) activity. By Northern blot analysis, this study provedthat asingletreatmentofmouse skin with BPO at dose of 40mg profoundly increased the level of ODC mRNA at about 6thhour after the BPO treatment The multiple treatment of mouse skin with BPO,once every two days for 4times,also elevated ODC mRNA level mainly at about 6th hour of last treatment These suggest that the induction of ODC activity by BPO is related to its stimulation of ODC mRNA expression.