多聚酶链反应检测乙型肝炎患者外周血单个核细胞中的HBVDNA

DETECTION OF HBVDNA BY POLYMERASE CHAIN REACTION IN PERIPHERAL BLOOD MONONUCLEAR CELLS IN PATIENTS WITH HEPATITIS B

本文应用多聚酶链反应（ＰＣＲ）技术检测了６９例乙型肝炎患者（其中慢性迁延性肝炎１７例，慢性活动性肝炎２３例，肝炎后肝硬化１２例，重症乙肝１７例）外周血单个核细胞（ＰＢＭＣ）中的ＨＢＶＤＮＡ存在状态。结果显示，ＰＢＭＣ中ＨＢＶ－ＤＮＡ总阳性率为４６％，而各型乙肝间ＰＢＭＣ中ＨＢＶＤＮＡ阳性率差异无显著性（Ｐ＞０．０５），提示ＨＢＶ对ＰＢＭＣ的感染，似与乙型肝炎的临床类型无明显的相关性。

HBV DNA in peripheral blood mononuclear cells(PBMCS) in 69 patients with hepatitis B was detected by polymerase chain reactions(PCR).The results showed that the total HBV DNA positive rate in PBMCS was 46%.No differences were found among different clinical types of hepatitis B in HBV DNA positive rate of PBMCS. It is indicated that the HBV DNA positive rate was not related to clinical types of hepatitis B.