顺磁金属离子与某些氨基酸分子络合的NMRT＿1法研究

THE STUDIES ON PARAMAGNETIC IONS COORDINATED TO SOME AMINO ACIDS BY NMR T_1 METHOD

本文研究了水溶液中微量顺磁离子Ｆｅ￣（３＋）和Ｃｕ￣（２＋）对蛋白质及多肽结构单元一氨基酸分子纵向弛豫时间Ｔ＿１的影响，把文献已处理过的金属离子与分子只存在一种络合物种体系的公式，推广到了多个络合物种并存且存在交换的体系中，导出并验证了顺磁弛豫速率Ｒ＿ｆ。与金属离子浓度Ｃ＿Ｍ成线性关系，较深入地讨论了斜率大小与络合能力、配体浓度的关系．

The influence of paramagnetic ions， Fe^(3＋) and Cu^(2＋) on relaxation time T_1 of aminoacids which are structural units of peptides has been investigated in this paper .The for－mula only for monospecies system of chemical molecule and metal ion has been ex－tended to polyspecies system and the system in which there are chemical exchanges． Thelinear relations of paramagnctic relaxation rate（R_(1p)）and concetration of metal ions（C_M）have been elucidated and demonstrated．The relations between the slope of curvesand coordinating ability and concentration of ligands have been discussed。