PCR方法检测我国脊髓灰质炎病毒Ⅰ型野毒株的研究

SURVEILLANCE OF WILD POLIOVIRUS TYPE 1 IN CHINA

用聚合酶链反应试验（ＰＣＲ）检测我国脊髓灰质炎病毒Ⅰ型（ＰＶＩ）野毒株，仅需５μｌＰＶＩ细胞培养液，方法简便、快速、敏感、特异，用本法对我国１４个省市７９份ＰＶＩ分离株的检测结果，能与检定ｓａｂｉｎⅠ相关株的ＳＩ／ＰＣＲ法的检测结果相印证，与其中３１份核苷酸测序判断为野毒株的结果相一致。其检测阳性率为８４．４％，基本能检测我国流行过的５个ＰＶＩ基因型野毒株。另外，从检测结果看，除北京市未发现野毒株外，其它１３个省区都有野毒株流行，表明当前我国消灭脊灰的任务还很重。

poliovirus typeⅠ(PV Ⅰ)wild strain-specific polymerase chain reaction (Wl/PCR)was developed for detection of wild PVI from China.The Wl/PCR method is easy to operate,sensitive and precise by using only 5 μl of PVI tissue culture medium for assay.The resultsof 79 PVI isolates from 14 regions in China examined by both PVI wild strain-specific and Sabin-specific primers were confirmed each other and were coincided with the identification of 31 wild strains by partial nucleotide sequencing.The method can roughly detect all 5genotypes of wild PVI circulated in hese regions,accounting for positive rate of 84.4%,exceptBeijing with no wild strain .It implys that polio eradication in China is not an easy work.