摘要
应用重组痘苗病毒作载体,在ATI、P7.5突变型早期启动子控制下,高效表达了(经定点突变去除一处早期终止信号的)HIV-1env基因。经Westernblot证明,晚期启动子活性强的pSFJ2-38能更高效地表达env基因。经Lentil-Lectin提取以及SDS-PAGE后的激光扫描测定结果,在107个感染细胞中可产生50-70μg的Env蛋白。
recombinant vaccinia virus was used as vector.Under control of ATI and P7.5 mutant early promotor,the HIV-I env gene,deprived of an early terminal signal,was successfullyexpressed to high level.The result of Western blot showed that the late promoter with highactivity,pSFJ2-38,induced higher expression of env gene.After preliminary purification with Lentil-Lectin and SDS-PAGE treatment,estimation with laser scanning technique indicated that 50-70μg of Env protein could be produced in 107 infected cells,the highest production of HIV-I Env protein in cell cultures ever reported.
出处
《病毒学报》
CAS
CSCD
北大核心
1994年第4期327-333,共7页
Chinese Journal of Virology