高脂血清对易感和不易感动脉粥样硬化动物的动脉平滑肌细胞生长的影响

Effect of Hyperlipidemic Serum on Proliferation of Aortic Smooth Muscle Cells from Animals Susceptible or non-susceptible to Atherosclerosis

体外培养家兔和树鼩为主动咏平滑肌细胞,分别观察各自的高脂血清对平滑肌细胞生长的影构,以~3H-胸腺嘧啶核苷(~3H—TdR)掺入细胞DNA,用液体闪烁计数器计数。结果表明:兔高脂血清对兔平滑肌细胞生长有显著的增殖作用,而树鼩高脂血清对树鼩平滑肌细胞的生长未见显著的促进增生作用。

It is known that rabbits are susceptible and Tree Shrews are resistant to the development of atherosclerotic lesions. Cultivated smooth muscle cells originated from the thoracic aortae of both animals were used respectively in studying the effecte of hyperlipidemic sera on the proliferation of the above cells. A concentration of 5 % hyperlipidemic serum in media was selec ted for by feeding with a high cholesterol diet and the effect on proliferation were measured with liquid Scintillation counter by incorporating the cultivated cells with 3H-TdR.The results showed that addition of rabbits hyperlipidemic serum gave a more significant increase of proliferation rate of smooth muscle cells than that induced by normal serum, however, the preliminary data showed that hyperlipidemic serum of Tree Shrews gave no significant effect on the proliferation of smooth muscle cells.