Sm抗原纯化及ELISA检测Sm抗体的初步研究

Preliminary Studies on Purification of Sm Antigen and Detection of Sm Antibody by ELISA

从小牛胸腺中盐析出来的Sm/RNP提取物经DEAE Bio-Ge1 A层析即为Sm抗原。在PAGE检测时Sm抗原在BSA之前有几条染色带。 血清学研究显示,Sm抗原仅与Sm阳性和Sm/RNP阳性血清发生反应,不与RNP阳性血清发生反应。经Sm抗原吸收后,Sm阳性和Sm/RNP阳性血清与Sm抗原的反应降低至接近正常人水平,而上述血清与RNP抗原的反应设有明显降低。结果表明,Sm与RNP已完全分开。ELISA方法检测Sm抗体有10/18的SLE和2/4MCTD是阳性,在14例RA、硬皮病和其它疾病中为阴性。

Sm antigen was purified by DEAE Bio-Gel A Chromatography from crude preparation of Sm/RNP that was salted out from calf thymus powder , there is a major band ahead of BSA when the purified Sm antigen was analysed with PAGE.The serological studies showed that the Sm antigen was only reacted with Sm positive and Sm/RNP positive sera, but not with RNP positive or normal serum.After absorption with Sm antigen, the reaction of Sm positive serum and Sm/RNP serum to Sm antigen dechied to about normal, the reaction of RNP positive serum and Sm/RNP positive serum to RNP were not decreased re-markedly.The results indicate that Sm has been separated from RNP completely. Using the Sm as antigen in ELISA, Sm antibody were positive in 10/18 SLE, 2/4 MCTD and negative in 14 cases of RA, scleroduma and other diseases.