豚鼠胸腺与骨髓细胞混合培养体外造血的研究

Hemopoiesis in vitro of mixed culture of bone marrow cells and thymus cells in guinea pigs

本文对豚鼠胸腺与骨髓细胞混合培养,及其体外造血、上悬液中成纤维细胞集落生成细胞(FCFC)的再次培养,底层贴壁的基质祖细胞(CFU—F)分泌成集落刺激因子(CSF),粒一巨噬系祖细胞(CFU—GM)培养等进行了初步研究,并对胸腺与骨髓混合培养体外违血的作用机制进行了观察和讨论。

Hemopoiesis in vitro of mixed culture of bone marrow cells (BMC) and thymus cells (TC) in guinea pigs was studied. The number of CFU-F colo-nies in the experimental group increased more sharply than that of the control group. When CFU-F of supernatant of the above mixed cultre medium was recultured, the CSF activity induced in these supernatants stimulated the CFU-GM more strongly than that of the separately cultured. It was believed that CFU-GM and CFU-F in this mixed culture medium might be Persistent at least for 12 days or more.