抗体固定在尼龙网上的研究

Research for Immobilization of Antibodies on Nylon Net

本文报道用尼龙网作载体,用抗乙型肝炎表面抗原作模型抗体,采用三种不同方法制作抗体膜,并在理论上进行了讨论.结果证明用硫酸二甲酯,1,6—己二胺和戊二醛制作的抗体膜最适于组装酶免疫传感器.因为它具有机械强度高、抗体结合量大,空间位阻小和气液通透性好等特点.酶免疫传感器的响应时间和电极上没有抗体膜时对酶反应产物的响应时间一样.

The antibody-bound membranes prepared by the three methods with anti-HBsAg was used as a model antibody and nylon net as a support. In the three mem-branes resulted, the membrane prepared with dimethyl sulphate, 1. 6-hexanediamineand gluteraldehyde is the most suitable to construct enzyme immunosensor because itcharacterized by high mechanical strength, high binding capacity of antibody, smallsteric hindrance and good permeability for gas and liquid. It also has an excellent re-sponse time, matching the clectrode respones time for the product of the enzyme reac-tion without the antibody-bound membrane.