马铃薯无菌苗叶肉原生质体再生植株

PLANT REGENERATION OF MESOPHYLL PROTOPLASTS FROM AXENIC SHOOT OF POTATO (SOLANUM TUBEROSUM L.)

商用马铃薯叶肉原生质体,在不同的液体培养基中浅层培养,再生细胞分裂,并获得愈伤组织。将愈伤组织转到固体培养基上诱导分化,已从克新四号和68-62两个马铃薯品种的原生质体得到再生的完整植株。再生细胞的分裂频率受原生质体培养密度的影响。细胞团的生长对液体培养基中的蔗糖浓度敏感。不同的原生质体培养基对愈伤组织的分化频率的影响非常显著。在分化培养基中加入3%的甘露醇,能提高愈伤组织的分化频率,并缩短分化期。

Mesophyll protoplasts of commereial potato were cultured with liquid medium as shallow layer. Regenerated cells divided and the calli were obtained. The regeneration plants of two potato lines (Ke Xin 4; 68-62) had been obtained from the regenerated calli after transferring onto the solid medium.The division frequency of regenerated cells was influenced by the culture density of protoplasts. The growth of protoplast-derived colony was sensiitve to the concentration of sucrose in liquid medium. The protoplast culture medium obviously influenced on the differentiation frequence of protoplast-derived calli(P-calli).After the supplement of 3% mannitol into the differentiation medium, the differentiation frequency of P-calli was improved and the differentiation period of P-calli was shortened.