摘要
从体外培养的恶性疟原虫中分离纯化总mRNA,将其逆转录为cDNA,组入表达型载体λgt11,建立了恶性疟原虫中国海南岛分离株FCC1/HN红内期cDNA库。经大肠杆菌表达后用抑制性单克隆抗体M26-32、F6-C2、F6-D3筛选。杭有27个克隆与M26-32作用、34个克隆与M26-32和F6-C2都反应。对筛选结果进行了分析。这些阳性克隆的表达产物有可能作为疟疾疫苗的组成部分。
Plasmodium falciparum HAINAN isolate FCC1/HN was cultured in large quantities in vitro. Total mRNA was purified from parasites and reverse transcribed into cDNA. By inserting eDNA fragments into λgt11, a P. falciparum FCC1/HN erythroeytic stage cDNA library had been constructed. 3 inhibitory McAbs M26-32, F6-D3 and F6-C2 were used for screening the cDNA library expressed in E. coli. So far, 27 positive clones have been found to react with M26-32 alone; and 34 clones to react with both M26-32 and F6-C2 McAbs. These expressed proteins might be used as candidates of malaria vaccine.
出处
《生物工程学报》
CAS
CSCD
北大核心
1989年第2期92-96,共5页
Chinese Journal of Biotechnology
关键词
疟原虫
cDNA库
单克隆抗体
Plasmodium falciparum
erythrocytic stage
cDNA library
McAbs
