利用微生物生产丙烯酰胺的研究

STUDIES ON PRODUCING ACRYLAMIDE UTILIZING MICROORGANISMS

丙烯酰胺主要用于生产聚丙烯酰胺。聚丙烯酰胺是一种用途十分广泛的精细化工产品,可以制成絮凝剂用于废水处理,也可以制成纸浆增强剂用于造纸工业,近年来,聚丙烯酰胺取代了天然植物胶而应用于原油的深度开采。丙烯酰胺的生产工艺早期采用硫酸法,因存在产品精制困难,环境污染等问题而被淘汰。近年来,美。

Pseudomonas putida JP-1 has nitrile hydratase which hydrates acrylonitrile into aerylamide. The condition for the producing nitrile hydratase of the strain JP-1 and the properties of nitrile hydratase in intact cells of the strain JP-1 have been investegated. The results suggested that the strain JP-1 can utilize propionitrile as source of carbon and nitrogen. Additive corn steep liquor is marked factor for enzyme formation. The optimum pH of nitrile hydratase was 7.5, optimum temperature was 15—30℃. When the reaction was carried out at 10℃, 21.5% acrylamide was produced in 4 h. The yield was more than 99% with less than 0.5% acrylic acid.