摘要
本文提出了以普通变形杆菌(Proteus vulgaris)发酵生产脲酶的方法。文中对发酵条件进行了系统的研究,使发酵得率达到每毫升培养基8.4u,时间仅需8h。同时还建立了提取和纯化的工艺,使能达到工业化生产水平。临床应用证明该脲酶可代替巨豆脲酶,为脲酶的生产开辟了一条新的途径。
The present paper reports a process for production of urease by fermentation of Proteus vulgaris. The fermentation conditions, including formula of culture medium and its sterilization, temperature, pH, aeration, stirring rate etc. have been systematically studied. The yield reaches 8.4u per mililiter culture medium in 8h. The procedure of extraction and purification has been established, so it can be produced industrially. Urease produced by this method have been proved by clinical use that it may be able to replace the urease from Jack bean. This method proved a new approach for the production of urease.
出处
《生物工程学报》
CAS
CSCD
北大核心
1989年第2期135-139,共5页
Chinese Journal of Biotechnology
基金
国家“七五”重点攻关项目
关键词
普通变形杆菌
脲酶
发酵
Proteus vulgaris
urease
fermentation
