摘要
生物化学需氧量是目前国际上用来衡量水质污染程度的重要指标,但常规法操作繁琐,误差较大,并需5天时间。本文采用将异常汉逊酵母夹在醋酸纤维素酯膜与聚全氟乙丙烯膜之间的固定化方法(夹层法)制得微生物膜,将此膜与氧电极复合便组成BOD传感器,并采用流通式测定系统,经试验,得出最佳测定条件。发现电极的电流差值与标准废水的BOD之间存在显著的线性关系,其线性范围为1—45mg/L。应时间小于15min,电极的保藏寿命为一年以上,测定误差小于6%。
The biochemical oxygen demand (BOD) is one of the most important and widely used parameter in the measurement of organic pollution. The determination of BOD by conventional method takes 5 days, which is too long and complicated for the use in process control. In this paper, a microbial sensor consisting of immobilized yeast cells (Hansenula anomala) and an oxygen electrode was eleborated for the estimation of BOD. The structure of electrode was delicately designed and a flow-through measuring system was set up in this study. The response time of the biosensor is within 15 minutes. Alinear relationship was observed between the current decrease and BOD of the sample solution(GGA, model waste water) in the range of 1—45mg/L. The storage life of the sensor is more than one year. The relative standard deviatoin is less than 6% at concentration of 20mg/L BOD.
出处
《生物工程学报》
CAS
CSCD
北大核心
1989年第3期235-240,共6页
Chinese Journal of Biotechnology
关键词
生化需氧量
传感器
固定化
Microbial sensor
BOD sensor
immobilization
