水合氧化钛固定化蛋白水解酶在蛋白质肽图分析上的应用

AN APPLICATION OF HYDROUS TITANIUM OXIDE IMMOBILIZED PROTEOLYTIC ENZYMES TO PEPTIDE MAPPING

自1977年Cleveland等用数种蛋白水解酶水解两种蛋白,再用聚丙烯酰胺凝胶电泳进行肽段比较以来。

Hydrous titanium oxides immobilized trypsing, papain, B.Subtilis AS1.398 neutral protease were applied to the peptide mapping with good resolution on the PAGE gel. After protein digestion enzyme was removed easily by simply centrifugation, no disturbances were found on the gel by proteolytic enzymes. The procedure is simple without specific requirsites and it is also cheap.