早熟大豆外源DNA导入的RAPD分子验证

MOLECULAR VERIFICATION OF SOYBEAN FOREIGN PEA INTRODOCNON TO INDUCE EARLINESS THROUGH RAPD TECHNIQUE

利用花粉管通道技术直接导入外源总ＤＮＡ，从而进行农作物品种改良，在国内外许多作物上已得到了广泛的应用。但外源总ＤＮＡ是否能够通过花粉管通道进入受体，后代的变异是不是由于外源总ＤＮＡ片段与受体基因组整合、表达所引起的，一直没有得到直接的分子生物学证据，本文报道了利用ＲＡＰＤ（ＲａｎｄｏｍｌｙＡｍｐｌｉｆｉｅｄＰｏｌｙｍｏｒｐｈｉｃＤＮＡ）这一分子生物学技术，对通过花粉管通道导入外源总ＤＮＡ所获得的大豆早熟后代进行了分子验证。结果表明：在后代基因组中找到了供体具有而受体没有的特异性ＤＮＡ片段，直接从ＤＮＡ分子水平上证明了外源总ＤＮＡ片段可以通过花粉管通道导入受体，并与其基因组ＤＮＡ整合，在后代中得到表达和遗传。

The technology of intrduction foreign total DNA through pollen tube channel had been widely used on many kinds of crops' improvements.But this technology had never been verified through molecular biology.The question was whether total DNA can get into recipient through pollen tube channel or not, and whether the variations were caused by foreign total DNA fragments inserting into recipient and brought into expressingor not.Soybean early maturing progenies derived from introduction of foreign total DNA verified through molecular biology techniques-RAPD(Randomly Amplified PolymorphicDNA)is reported in this paper.The result denote that:The specific DNA fragments of donor have been found in genome of progenies and these fragments didn't exist in the original recipient.Foreign total DNA fragments can insert into recipient through pollen tube channel and perform phenotypic expression can be inherited in progenies.This have been directly verified through technique of molecular level of DNA.