大豆SMV病源相关蛋白的诱导与耐病性获得

INDNETION OF SOYBEAN SMV PATHOGENESIS-RELATED PROTEIN AND ACGUISTION OF FOLERANCE

本研究利用ＳＤＳ—ＰＡＯ技术，研究了不同抗病性品种（系）接种ＳＭＶ１号株系后，病源相关蛋白的表达变化。结果表明：大豆接种ＳＭＶ后，感病品种蛋白亚基增加数目较多，同时也伴随着一些正常表达的蛋白亚基的降解。而高抗ＳＭＶ１号病毒的大豆株系，接种ＳＭＶ病毒后，蛋白亚基增加１—２条，其正常工作的蛋白组分均没有消失，蛋白亚基消失与表达的变化与品种的耐病性获得相一致。这一结果，目前尚未见报道，深入研究病源相关蛋白的性质、表达和消失，对研究大豆感染ＳＭＶ和抗病性的分子鉴定具有一定的意义。

The changes of pathogenesis- related protein (PR-Protein) of different soybean cultivars (Lines) with different degree of resistame by inoculating with mosaic virus (SMV) trains were studied with SDS-PAG. After inocnlating with'SMV', The Protein subunit of susceptible varieties of soybean were more than that of resistant one, mean while some normal protein subunits were degraded. One or two protein subunits of the soybean lines resistant to SMV No. 1 increased in comparison with that of ck, but normel express protein subunits didn treduce. The change of degradation and appearition of the protein subunits was in accordance with the disease resistance of the cultivars. So tu, there is on report related about this result. A thoroush research on the characteristics appearance, and degradation of the PRprotein is very improtant for the undstanding of molecular festing technique for susceptibility and resistance of soybean to SMV.