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利用TLS提取染色体DNA 被引量:3

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摘要 本文报告利用三乙醇、胺月桂基硫酸盐代替蛋白酶提取组织、细胞及外周血DNN。方法简便、快速、经济。DNA产率、纯度、分子量等标准均不低于蛋白酶水解法,可以满足基因分析的要求。
出处 《生物化学与生物物理进展》 SCIE CAS CSCD 北大核心 1989年第3期221-223,共3页 Progress In Biochemistry and Biophysics
关键词 TLS 染色体 DNA提取
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  • 1马旭,张思仲,赵丽.孕妇血中胎儿细胞性质和来源的研究[J].中华医学杂志,1995,75(10):631-632. 被引量:16
  • 2Ville Y, et al. Are desquamated trophoblastic cells retrieved from the cervix suitable for a prenatal diagnosis? Contracept Fertil Sex, 1994Jul-Aug, 22(7-8) :475-7.
  • 3Massari A, et al. Non-invasive early prenatal molecular diagnosis using retrieved transcervical trophoblast cells. Hum Genet. 1996 Feb,97(2): 150-5.
  • 4Kingdom J, et al. Detection of trophoblast cells in transcervical samples collected by lavage or cytobrush. Obstet Gynecol. 1995 Aug, 86(2):283-8.
  • 5Lo YMD, Corbetta N, Chamberiain PF, et al. Presence of fetal DNA in Meternal plasma and serum. Lancet, 1997,350:485-487.
  • 6Hamada H, Arinami T, Hamaguchi H, et al. Fetal cells in maternal peripheral blood after delivery. Am J Obstet Gynecol, 1994, 170:1188.
  • 7Steele CD, et al. Prenatal diagnosis using fetal cells isolated from maternal peripheral blood: a review. Clin Obstet Gynecol, 1996 Dec, 39(4) :801-13. Review.
  • 8Suzumori K. The role of fetal blood sampling in prenatal diagnosis.Early Hum Dev, 1992 Jun-Jul, 29(1-3): 155-9.
  • 9Al-Mufti R, et al. Investigation of maternal blood enriched for fetal cells:role in screening and diagnosis of fetal trisomies. Am J Med Genet, 1999 Jul 2, 85(1) :66-75.
  • 10Troeger C, et al. A comparison of different density gradients and antibodies for enrichment of fetal erythroblasts by MACS. Prenat Diagn, 1999 Jun, 19(6):521-6.

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