氯喹对伯氏疟原虫摄取次黄嘌呤、甲硫氨酸、精氨酸及其氨基酸组成的影响

Effects of Chloroquine on Incorporation of Labelled Compound and on Cotents of Amino Acids in Plasmodium berghei

伯氏疟原虫氯喹敏感株和抗氯喹株感染的RBC,与0.4mmol/L氯喹一起培养2小时后,敏感和抗氯喹株感染的RBC,对[~3H]次黄嘌呤、[^(14)C]精氨酸和[~3H]甲硫氨酸的摄入量分别被抑制67.3％、41.8％和35.7％以及65.4％、45.6和46.9％。 感染疟原虫的小鼠,经氯喹10mg/kg肌注20小时后,各氨基酸组成,在敏感株疟原虫中普遍的较不服药的对照组上升,而在抗氯喹疟原虫中,升高的氨基酸主要是与多胺、谷胱甘肽有关,如精氨酸、鸟氨酸、甲硫氨酸、脯鼠酸、甘氨酸和半胱氨酸。

The result showed that [G-3H] hypoxanthine, [14C] arginine, [3H] methionine, [14C] glucose and [5-3H] orotic acid were taken by the RBC infected with P.berghei of both chloroquine (CQ)-sensitive and CQ-resistant strains. After treatment with CQ(0.4mmol/L), the incorporation of [3H] hypoxanthine [14C] arginine and [3H] methionine were inhibited, their inhibitory rates were 67.3, 41.8 and 35.7% in CQ-sensitive and 65.4, 45.6 and 46.9% in CQ resistant strain respectively.20h after intramuscular injection of CQ(l0mg/kg)to the mice infected with strains of P.berghei, the contents of most amino acids increased in CQ-sensitive group, whereas in the resistant group methionine, arginine, ornithie, proline, gly-cine and cysteine, which were relevent to the synthesis of polyamines and guta-thion, increased significantly.