竹红菌甲素敏化质粒pBR 322 DNA光氧化——使封闭环DNA转变为开环DNA

Hypocrellin A-sensitized Pnotooxidation of pBR 322 DNA Resulting in Strand Scission to Open Circular DNA

甲素可敏化质粒pBR 322 DNA光氧化断链的使其封闭环DNA转变为开环DNA。甲素敏化pBR 322 DNA光氧化反应可被单线态氧淬灭剂-NaN_3抑制,证明此光敏氧化机制属Ⅱ型过程。

The photooxidation of pBR 322 DNA sensitized by Hypocrellin A resulted in the conversion of closed circular DNA to open circular DNA. This result showed that strand scission of pBR 322 DNA might occur in this photooxidation. Singlet oxgen quencher-NaN3 could protect DNA from strand scission suggesting that Type Ⅱ process was involved in Hypocrellin A-sensitized photooxidation of pBR 322 DNA.