ADP-核糖基转移酶的抑制对γ线所致肿瘤细胞DNA损伤的加强作用

Enhancement of THE DNA Damage in γ-RAY Irradiated Tumor Cells by Inhibition of ADP-Ribose Transferase

本文探讨了ADP—核糖基转移酶(ADPRT)的活性、DNA单链断裂(SSB)重接和细胞潜在致死质损伤修复(PLDR)三者的关系。证明了ADPRT的特异性抑制剂3—氨基苯甲酰胺(3AB)能阻抑γ线所致的小鼠腹水瘤细胞DNA SSB的重接和PLDR。为增强放射治疗的效果提供了可能的新途径。

In this paper a close relationship among the activity of ADP-ribose transf-erase (ADPRT), rejoining of DNA single strand breaks (SSB) and potentially lethal damage repair (PLDR) in mouse tumor cells has been demonstrated. When the cells were treated with 3-aminobenzamide (3AB), a specific inhibitor of ADPRT, and subjected to γ-ray irradiation at a dose of 6 Gy, the decrease of intracellular NAD was retarded. Consequently, the rejoining of DNA ssb was inhibited,the PLDR of the cells was depressed, and the survival fraction of tumor cells sharply decreased. These results might suggest a possible way to increase the efficiency of cancer radiotherapy.