四种弓形虫病血清学诊断方法的比较研究

A Comparison Study on Four Serologic Methods in Clinical Diagnosis and Mass Survey of Human Toxoplasmosis

本研究应用弓形虫的斑点酶联免疫吸附试验（Ｄｏｔ—ＥＬＩＳＡ）、常规酶联免疫吸附试验（ＥＬＩＳＡ）和间接免疫荧光试验（ＩＦＡ）及间接血凝抑制试验（ＩＨＡ）四种方法，分别检测了同一批人血清（１５０份）。结果表明：（１）四种方法中以Ｄｏｔ—ＥＬＩＳＡ最为敏感，血清阳性检出率最高为１９．３％；ＩＦＡ次之为１１．３％；ＥＬＩＳＡ为８．６９％；ＩＨＡ最低仅为６．６６％。（２）Ｄｏｔ—ＥＬＩＳＡ明显优于常规ＥＩ，ＩＳＡ、ＩＨＡ、ＩＦＡ，具有敏感、特异、简便、经济等优点，值得推广应用。

n order to select a more sensitive and specific serologic experimental method for clinical diagnosis and 0n- the-spot-check of human toxoplasmosis,a synchronous experimental comparison study was carried out on four serologic tests(Dot ELISA,ELISA,IFA and IHA)for human toxoplasmosis,150 homosera from Xin-jiang were tested with those methods,and results showed that DOt ELISA with definite specificty was the most sensitive than the other tests ,and that its detectable rate of the disease was 19.3%, while the others were 11.2%(IFA),8.69%(ELISA)and 6.66%(IHA),respectively。Therefore, in our opinion,Dot ELISA could be used widely in the clinical diagnosis and mass survey for human toxoplasmosis。