紫外分光光度法测定兔血清中二氯乙酸钠浓度

Determination of serum sodium dichloroacetate content in rabbits us-ing ultraviolet spectrophotometry

作者建立检测二氯乙酸钠血清浓度的紫外分光光度法，平均回收率为１０１．４％，日内变异为３．３％，日间变异为３．８％，最低检测浓度为１ｍｇ／Ｌ．应用本法研究二氯乙酸钠（ｉｖ，１８０ｍｇ／ｋｇ）在兔体内的药代动力学，主要药代动力学参数为：Ｔ１／２＝０．６７ｈ，Ｖｄ＝０．２８Ｌ／ｋｇ，Ｋ＝１．１ｈ－１，Ｃｌ＝０．３１Ｌ／ｋｇ／ｈ，ＡＵＣ＝６０５．１ｍｇ·ｈ／Ｌ．

The authors determined serum sodium dichloroacetate(DCA-Na)content in rabbits using ultravio-let,spectrophotometry with a wavelength of 265 nm.The mean recovery rate was 101.4%,the intra-day and inter-day cv was 3.3% and 3.8%,respectively,and minimal detectable level was 1 mg/L.Pharmacokinetic study on DCA-Na was carried out in rabbits receiving a single intravenous injection of DCA-Na (180 mg/kg).The changes in serum DCA-Na concentration were best described by a 1-compartment open model using PKBP-N1 program.The main parameters obtained were as follows:T1/2 0.67 h,Vd0.28 L/kg,Cl0.31 L/ kg/h and AUC 605.1mg·h/L.