阿霉素和丝裂霉素能诱导接种于裸鼠人肝癌组织中抗药基因的表达

Adrimycin and Mitomycin C Induced Overexpression of MDR_1 Gene in Human Hepatocellular Carcinoma Tissue-grafted Nude Mice

作者观察了阿霉素和丝裂霉素对接种于裸鼠人肝细胞癌组织中抗药基因表达的影响。结果显示：阿霉素治疗组４例中有３例在化疗后两周就有ＭＤＲ１ｍＲＮＡ表达增加，这种增加在化疗后４周更明显；丝裂霉素治疗组５例中有３例在化疗后两周有ＭＤＲ１ｍＲＮＡ表达的少量增加，但在４周时ＭＤＲ１ｍＲＮＡ表达显著提高，另外２例ＭＤＲ１基因表达在化疗前后无明显变化。结果提示：阿霉素和丝裂霉素可能通过诱导ＭＤＲ１基因的过量表达而使人肝癌细胞产生抗药性。

In this study, we observed the effects of adrimycin and mitomycin C on the inducing of multidrug resistant gene 1(MDR1) expression in human hepatocellular carcinoma tissue-grafted nude mice. Two week-old nude mice was transplanted with human hepatocellular carcinoma tissue.The nude mice were treated by injection of adrimycin and mitomycin C into the abdomen when the tissues were 8～10 mm in diameter. The results are as follows. (1) Overexpression of MDR1 gene was observed in 3 out of 4 hepatoma tissues treated with adrimyicn after 2 weeks, obviously after 4 weeks. (2) Overexpression of MDR1 gene was also observed in 3 out of 5 hepatoma tissues treated with mitomycin C after 4 weeks. These results indicate that adrimycin and mitomycin C can induce the MDR1 gene expression which leads to acquired drug resistance of human hepatocellular cancer.