白细胞介素－1α诱导牛脑微血管平滑肌细胞增殖及药物的拮抗作用

Interleukin-1 Alpha Induced Proliferation of Bovine Cerebromicrovascular Smooth Muscle Cells and Antagonism of Drugs

作者研究了白细胞介素－１α（Ｉｎｔｅｒｌｅｕｋｉｎ－１α，ＩＬ－１α）对培养的牛脑微血管平滑肌细胞增殖的影响。在５～５００ｐｇ·ｍｌ－１范围内，ＩＬ－１α可诱导培养的牛脑微血管平滑肌细胞增殖，当用５０ｐｇ·ｍｌ－１ＩＬ－１α与该平滑肌细胞共孵育２４ｈ时，刺激作用最强，增殖率达２９．１％。欧芹素乙、异欧芹素乙，６－（α，α－苯基乙酰哌嗪基苯基）－４，５－二氢－５－甲基－３（２Ｈ）哒嗪酮在浓度为１０－６～１０－４ｍｏｌ·Ｌ－１时，可剂量依赖性地拮抗ＩＬ－１α诱导的该平滑肌细胞增殖，药物浓度最高（１０－４ｍｏｌ·Ｌ－１）时，抑制率值最大；６－（α，α－二苯基乙酰哌嗪基苯基）－４，５－二氢－５－甲基－３（２Ｈ）哒嗪酮，在低浓度时（１０－６和１０－５ｍｏｌ·Ｌ－１）可抑制ＩＬ－１α诱导的该平滑肌细胞增殖，药物浓度升高，抑制率也加大，而在高浓度时（１０－４ｍｏｌ·Ｌ－１）抑制率下降。

The effects of Interleukin-1 alpha(IL-1α) on the proliferation of bovine cerebromicrovascular smooth cells(BCSMC) were investigated. At concentration from 5 to 500 pg·ml-1, IL-1α induced proliferation of cultured BCSMC. At 50 pg·ml-1, after 24 h incubation with BCSMC, stimulative effect of IL-1α reached maximum: 29.1%. At concentration from 10-6～10-4 mol· L-1, imperatorin(Imp), iso-imperatorin(Isi) and 6-(α,α-phenylacetylpiperazinyl) phenyl-5-methyl-4,5-dihydro-3(2H)-pyridazinone(PMDP) antagonized IL-1α induced proliferation of BCSMC in a dose-dependent manner. At 10-4 mol·L-1, inhibitory rates of Imp, Isi and PMDP were respectively 62.1±2.1%, ;48.3±5.2% and 46.5±13.1%. At 10-6 and 10-5 mol.·L-1,6-(α,α- diphenylacetylpipezinyl) phenyl-5-methyl-4,5-dihydro-3(2H)-pyridazinone(DMDP) inhibited IL-1α- induced proliferation of BCSMC, and their inhibitory rates were respectively 34.3±3.5% and 56.6± 9.8%. At 10-4 mol·L-1, inhibitory rate of DMDP reduced by 28.3±4.6%.