摘要
本文报告烧伤焦痂提取物中可溶于有机溶剂组分D1对大鼠肝线粒体功能及呼吸链的损害作用。结果表明第3态呼吸受D1抑制,第4态受D1刺激而增高,耗氧率变化与D1用量之间存在着显著的量效关系。低剂量时耗氧率的改变缓慢,高剂量时耗氧率的变化急剧,这个转折点在D1浓度为1mg/ml处。氰化羰酰-3-氟苯腙(FCCP)/第4态耗氧率比值随D1浓度增加而下降,磷酸化位点1底物与位点2底物的实验结果相似,在D1浓度为1mg/ml处也存在一个比值下降坡度变化的转折点。FCCP/第4态耗氧率比值下降表示复合物Ⅰ~Ⅲ及Ⅱ~Ⅲ节段有损伤。与此相反,位点3底物实验中未见FCCP/第4态耗氧率比值变化,说明复合物Ⅳ无损伤。有关酶活力测定进一步证实上述呼吸链损伤的定位结果。受D1作用后,还原型辅酶Ⅰ(NADH):细胞色素c还原酶与琥珀酸:细胞色素。还原酶活力均明显下降,但复合物Ⅰ中的NADH脱氢酶与复合物Ⅱ中的琥珀酸脱氢酶活力却无改变,说明损伤的部位很可能在复合物Ⅲ即bc1complex处,有关的功能实验正在进行中。
The effects of substance D1,an extract from burn eschars,on hepatic mitochondrial respiratory function and the damages to the respiratory chain were studied in the rat.The rate of state 3 and state 4 respiration,the FCCP-stimulated respiratory rate,the respiratory control ratio,and the ratio between FCCP rate and state 4 rate were determined and the activities of NADH:cytochrome c reductase,succinate: cytochrome c reductase,NADH dehydrogenase and succinate dehydrogenase were measured.It was found that D1 inhibited state 3 respiratory but stimulated state 4 respiration. There was a dosage effect relationship between the dosage of D1 and the changes of the rate of Oxygen consumption. The turning point was at the D1 concentration of 1mg/ml.The ratio of FCCP-stimulated rate with state 4 rate was decreased when the dosage of D1 increased.The experimental results were similar if glutamate,malate or succinate was used.The turning point of the ratio decreasing gradient was at the D1 concentration of 1 mg/ml.The decrease of FCCP-stimulated rate and state 4 rate indicates that there are damages to the respiratory chain. Cytochrome oxidase,the terminal segment of the chain,was intact.Therefore,the damage might occur somewhere in complexes Ⅰ~Ⅲ and Ⅱ~Ⅲ.The activities of NADH:cytochrome c reductase and succinate:cytochrome c reductase were both markedly inhibited while those of NADH dehydrogenase and succinate dehydrogenase remained intact. These findings suggest further that the damage might occur in complex Ⅲ,the bc1 complex.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1994年第1期1-4,共4页
Journal of Third Military Medical University
基金
国家自然科学基金
关键词
焦痂
线粒体
呼吸链
脱偶联
烧伤
burns
eschar
mitochondria,liver
respiratory chain
phosphrylation site
uncoupling