人肿瘤坏死因子α基因cDNA直接注入小鼠骨骼肌表达的初步研究

A preliminary study of the expression of TNFαgene cDNA through direct gene transfer into skeletal muscles in mice

本实验将带有人肿瘤坏死因子。基因（ＴＮＦ－α基因）ｃＤＮＡ的重组表达质粒直接注入小鼠骨骼肌内，观察了外源基因在小鼠体内的表达情况。双抗体夹心法ＥＬＩＳＡ法显示，实验组小鼠肌注１５ｄ后血浆中ＴＮＦ蛋白含量逐渐增高并达高峰，然后开始下降。ＲＮＡｄｏｔｂｌｏｔ结果显示，肌注２０ｄ时实验组中ＥＬＩＳＡ阳性的小鼠，其肌注处肌肉组织有ＴＮＦ－α基因表达。由此表明，直接将外源ＤＮＡ导入骨骼肌细胞内是基因转移的又一新途径。

It was found that an injection of the recombinant TNFαcDNA plasmid directly into the skeletal muscle of mice resulted in a significant expression of TNF gene in the muscular cells. Human TNF protein could be detected in the mice 15 days after injection with ELISA and RNA dot blot. It is concluded that direct introduction of exogenous DNA into the cells of skeletal muscles will be a new route for gene transfer.