大鼠脑挫伤后脑不同部位细胞和线粒体内的钙含量变化

Study of the effects of cerebral contusion on cellular and mitochondrial calcium level of different regions of the brain in rats

用原子吸收分光光度法（ＡＡＳ）及比色法分别测定了脑挫伤后８ｈ大鼠各脑区细胞内钙（Ｃａｉ）、组织总磷（Ｐｔ）、线粒体钙（Ｃａｍｔ）、线粒体（Ｐｍｔ）的含量变化，结果表明原发损伤可使多数脑区的细胞受到影响并发生Ｇａ２＋内流，局部伤情影响着Ｇａ２＋内流程度；内流Ｃａ２＋多是以结合形式沉积于胞浆等部位以维持胞内Ｃａ２＋浓度（［Ｃａ２＋］ｉ）稳定，蛋白质是可能的主要结合剂；但严重的Ｃａ２＋内流将造成线粒体钙储积及［Ｃａ２＋］ｉ失衡并引发组织细胞继发性破坏，导致Ｐｔ下降。

The cellular and mitochondrial level of calcium, the total phosphorus in brain tissue and mitochondrial phosphorus in different regions of the brain were determined with absorption spectroscopy and chromometry in rats 8 hours after they were inflicted with cerebral contusion. It was found that primary cerebral contusion could induce Ca influx of the cells in most of the brain regions and the intensity of Ca influx was correlated with the severity of local brain damage. Most of the influx Ca existed in the cytoplasm in bound forms to maintain a stable level of intracellular calcium ions, and protein was the main substance to bind with Ca. Massive Ca influx could lead to sequestration of Cain the mitochondria and disturbed intracellular calcium level.which in turn brought about secondary damages on the tissue cells and a decrease of tissue phosphorus.