烫伤大鼠肝葡萄糖激酶、肌己糖激酶活性及红细胞三磷酸腺苷含量的变化

Changes of hepatic glucokinase, muscular hexokinase, blood glucose,plasma lactate and erythrocytic ATP after scalding in rats

为进一步探讨损伤后糖代谢状况，我们测定了３０％体表烫伤后大鼠不同时间的血糖和血乳酸，并比较休克期（伤后６ｈ）和高代谢期（伤后７２ｈ）的肝葡萄糖激酶及比目鱼肌已糖激酶（Ⅱ型）活性。结果显示血乳酸水平在伤后６ｈ和４８ｈ各有一个高峰，可能分别是应激和代谢紊乱的表现。血糖水平在伤后６ｈ明显升高，但６ｈ内波动很大。伤后７２ｈ肝葡萄糖激酶活性显著降低（Ｐ＜０．０５），可能与创伤后血糖升高，糖异生增强及胰岛素抵抗等现象有关。伤后比目鱼肌已糖激酶活性及红细胞三磷酸腺苷（ＡＴＰ）含量未发现明显变化。

The concentration of blood glucose and plasma lactate were determined in rats after they were inflicted with 30%TBSA full thickness scalding. The activity of hepatic glucokinase (GK)and hexokinase (HK)type Ⅱ in the soleus in the shock phase(in the 6th h postinjury)was compared with that in the hypermetabolic phase(in the 72nd h postinjury). 2 peaks of plasma lactate concentration were found in the 6th and 48th h postinjury, which may be attributed to burn stress and metabolic disturbance respectively. The activity of hepatic GK was significantly decreased in the 72nd h postinjury( P<0.05), which might result from hyperglycemia, increased glycogenosis and insulin resistance. No significant changes of the activity of soleus HK and erythrocytic ATP were found after scalding.