1，3－丁二烯多次吸入染毒后小鼠红细胞微核率的流式仪检测的研究

An investigation of the incidence of micronucleated erythrocytes after multiple inhalations of butadiene with flow cytometry in mice

在本研究中使用作者新近建立的流式仪小鼠红细胞微核自动检测技术和微核常规检测技术，测定了小鼠连续吸入１，３－丁二烯气体５ｄ后（５０，２００，５００和１３００ｍｇ／Ｌ）骨髓和外周血红细胞的微核率变化情况，结果表明１，３－丁二烯能明显诱导小鼠红细胞微核率升高，其剂量反应为非线性增加，但曲线斜率随剂量增加而趋于平缓，由此证明１，３－丁二烯具有肯定的遗传毒性。同时小鼠外周血嗜多染红细胞率随剂量增大而下降，还说明该化合物具有细胞毒性。

The incidence of micronucleated polychromatic erythrocytes(MPCE)and normochromatic erythrocytes(MNCE)in the bone marrow and peripheral blood induced by multiple inhalations of l,3-butadiene was observed in mice with a new automatic flow cytometric technique designed by the authoors themselves and the conventional flow cytometry. 1,3-butadiene of 0,50,200,500 and l 300 ppm was inhalate by the mice repeatedly for 5 days.It was found that the incidence of MPCE and MNCE was significantly increased in both the bone marrow and peripheral blood after butadiene inhalation and the dosage-effect relation was non-linear.The slope of the curve was flattened when the exposure concentration of l,3-butadiene was increased.These findings suggest that l,3-butadiene possesses definite genetic toxicity and it also exerts cytotoxicity red cells since there was a decrease of MPCE in number in the peripheral blood when the dosage of l,3-butadiene was increased.