腮腺多形性腺瘤及恶性多形性腺瘤c－erbB－2癌基因扩增与患者预后的关系

Study of the relation between c-erbB-2 Protooncogene amplificationand prognosis in parotid pleomorphic adenoma and parotid malignantpleomorphic adenoma

作者采用ＤＮＡ斑点杂交方法，对３５例腮腺多形性腺瘤（ＰＡ）和３６例恶性多形性腺瘤（ＭＰＡ）石蜡包埋组织中ｃ－ｅｒｂＢ－２癌基因扩增情况进行研究．结果表明，３５例ＰＡ中，６例扩增（１７．１４％）；３６例ＭＰＡ中，２２例扩增（６１．１１％）；两组扩增有显著差别（Ｐ＜０．０１）．扩增组ＰＡ恶变率（８３．３３％）明显高于无扩增组（３．４５％，Ｐ＜０．０１）．各临床分期的ＭＰＡｃ－ｅｒｂＢ－２扩增率无显著差异（Ｐ＞０．０５），而不同分化程度的ＭＰＡｃ－ｒｅｒｂＢ－２扩增率有显著差异（Ｐ＜０．０５）．扩增组ＭＰＡ术后复发率明显高于无扩增组（Ｐ＜０．０１）；ＭＰＡ术后五年生存率无扩增组高于扩增组（Ｐ＜０．０１）．提示：ｃ－ｅｒｂＢ－２癌基因扩增可能成为ＰＡ及ＭＰＡ有价值的预后指标．

C-erbB-2 protooncogene amplification was analyzed with dot blot technique in paraffin-embeddedhuman tissues of 35 parotid pleomorphic adenomas(PA )and 36 parotid malignant pleomorphic adenomas(MPA).C-erbB-2 amplification was found 6/35(17.14%)in PA group and 22/36( 61.11%)in MPAgroup,amplifications in PA and MPA groups had highly statistically significant difference(P<0. 01 ).Therate of malignant change of PA with amplification was higher than that of without amplification(P<0.01 ).The amplification in MPA with different clinical stages had no significant difference(P>0.05).The amplifi～cations in MPA with different histologic differentiations showed significant difference(P< 0.05 ).The post-operative recurrent rate of MPA with amplification was significantly higher than that of without amplification(P<0. 01 ).The postoperative five-year survival rate of MPA with amplification was considerably lower thanthat without amplification(P<0.01).This observation indicates that c-erbB-2 amplification may be an use-ful independent prognostic indicator in PA and MPA.