摘要
Ｃ８杂交瘤分泌人的ＩｇＭ（λ）单克隆抗体，是具有肾综合征出血热病毒抗原内影像作用的Ａｂ２（β）我们用常规Ｇｉｅｍｓａ染色、Ｇ－分带和Ｃ－分带的系列染色法对Ｃ８杂交瘤细胞的染色体进行分析，发现除了小鼠染色体外，还含有人的１，２，５，７，９，１２，１６，１７，１８，１９，２０，２１和２２号染色体及大量标记染色体，人染色体中以较短的染色体出现频率较高。用系列染色法分析人－鼠杂交瘤中的染色体比单独用Ｇ分带法更易得到可靠的结论。
Hybridoma C8 secrets lgM(λ)human monoclonal antibody which has been identified to be an in-ternal image Ab2(β)of hemorrhagic fever with renal syndrome virus. We analysed the chromosomes in hy-bridoma C8 using serial conventional Giemsa,G- band and C-band staining methods and found that besidesmouse chromosomes,human chromosomes No. 1,2,5,7,9,12,16,17,18,19,20,21, 22 and manymarker chromosomes existed in this cell line. The occur rence frequencies of shorter human chromosomes arehigher than those of longer ones.
出处
《第四军医大学学报》
1994年第1期8-10,共3页
Journal of the Fourth Military Medical University
关键词
杂交瘤
染色体
免疫球蛋白
hybridomas
chromosomes
immunoglobulin
Giemsa staining
