肝缺血时铜、锌元素、氧自由基变化与“加味生脉散”的防治作用

Roles of free radicals and trace elements Cu,Zn in ischemic dog liver cell injury:Prevention of damage by″jia wei sheng mai san″

目的：为了探讨徽量元素铜、锌在肝缺血损伤中的作用及其与氧自由基的内在联系，并观察“加味生脉散”对急性肝缺血损伤的保护作用．方法：作者选用成年家犬，随机分２组，对照组术前３ｄ及术日前ｉｍ生理盐水０．４ｍｌ／（ｋｇ·ｄ），实验组则ｉｍ加味生脉散注射液０．４ｍｌ／（ｋｇ·ｄ）．复制活体犬肝缺血再灌注损伤模型，测定不同时间血、肝组织铜、锌含量．肝组织丙二醛（ＭＤＡ）变化及血中超氧化物歧化酶（ＳＯＤ）活性，ＡＬＴ变化及不同时间肝细胞形态改变。结果：随着缺血再灌注，肝组织内ＭＫＡ和血中ＡＬＴ含量逐渐升高，而ＳＯＤ活性及铜、锌含量逐渐下降，但术前应用加味生脉散，则显著地提高ＳＯＤ活性及铜、锌含量，降低肝组织及血中ＭＤＡ和ＡＬＴ含量，减少肝细胞变性，坏死的程度和范围，两组比较Ｐ＜０．０５～０．０１．结论：加味生脉散对肝缺血损伤显著的保护作用，为预防肝血损伤应给予自由基清除剂，也应给一定量的铜和锌元素．

Objective: TO determine whether Chinese medicine一“jia wei sheng mai san”(JWS) pretreatment could modify cellular free radical metabolism, rixe levels of Cu, Zn during hepatic ischemia and subsequent reperfusion and prolong the viability of liver. Methods: Total hepatic ischemia was induced with dogs. The JWS 0.4ml/(kg·d)and saline 0.4ml/(kg ·d)as a control were im for 3 days before ischemia was induced.Results: During hepatic ischemia and subsequent reperfusion, the MDA ALT increased markedly.The levels of Cu, Zn and SOD activity showed dramatic decreased(P<0.01).The pretreatment with JWS significantly attenuated ALT ,and also greatly suppressed the elevation of MDA and rised the levels of Cu,Zn and SODactivity (P<0. 05).Light microscopic feature of liver biopsy specimens showed that hepatic cellular degener-ation and necrosis were more marked in control than in treatment group. Conclusions: These results are com-patible with the assumption that cellular damage caused by hepatic ischemia can be explained by free radical riaction and suggest that administration of JWS as a free radical scavenger is effective in ischemic liver cell.